PCR fragments were amplified from the whole genome amplified seawater DNA.
Of these 55 clones contained conserved Pha C domains and were classified into 20 genetic groups depending on their sequence similarity.
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This pathway is integrated by the following: (i) a specific route (upper pathway), which catalyzes the conversion of 3-OH-Ph Ac into 2,5-dihydroxyphenylacetic acid (2,5-di OH-Ph Ac) (homogentisic acid, Hmg), and (ii) a central route (convergent route), which catalyzes the transformation of the Hmg generated from 3-OH-Ph Ac, -Tyr into fumarate and acetoacetate (Hmg ABC).
Thus, in a first step the degradation of 3-OH-Ph Ac requires the uptake of 3-OH-Ph Ac by means of an active transport system that involves the participation of a permease (Mha C) together with phosphoenolpyruvate as the energy source.
Once incorporated, 3-OH-Ph Ac is hydroxylated to 2,5-di OH-Ph Ac through an enzymatic reaction catalyzed by a novel two-component flavoprotein aromatic hydroxylase (Mha AB).
The large component (Mha A, 62,719 Da) is a flavoprotein, and the small component (Mha B, 6,348 Da) is a coupling protein that is essential for the hydroxylation of 3-OH-Ph Ac to 2,5-di OH-Ph Ac.
(Before Christ) have long been used by archaeologists in Europe and the Americas; their reference point is the birth of Christ.
(Before Current Era) are sometimes used instead of A.
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Douglas Raybeck and Stephanie Dorenbosch, anthropologists at Hamilton College, New York, worked with two psychologists, Michael Sarapata and Douglas Herrmann from Indiana State University, on the study.
Eleven genetic groups have undisclosed Pha C activity based on their distinct phylogenetic lineages from known PHA producers.